We published the following paper: "Liquid marble technology to create cost-effective 3D cardiospheres as a platform for in vitro drug testing and disease modelling." in the journal MethodsX.
Three-dimensional (3D) cell culturing has several advantages over 2D cultures. 3D cell cultures more accurately mimic the in vivo environment, which is vital to obtain reliable results in disease modelling and toxicity testing.
Current methods to achieve multicellular spheroids are expensive, time-consuming and require specialized materials and training. Hydrophobic powders can be used to create a micro environment for cell cultures, which are termed liquid marbles (LM).
In this procedure we describe the first use of the LM technology for 3D culturing in vitro derived human cardiomyocytes which results in the formation of cardiospheres within 24h. The cardiospheres could be used for several in depth and high-throughput analysis.
You can access the article by clicking here.